Friday, December 1, 2017

Enzyme lab report

Hypothesis: I think the temperature will affect the way our enzyme performs. If the temperature is high production will speed up. If the temperature is lower there will be a low production.



Independent Variable: Temperature change.



Dependent Variable: How much foam come out of the heated or cooled substrate when it is mixed with the plant extract(enzyme).



Controlled Variables: The enzyme at room temperature.



Justification of hypothesis: When stuff becomes heated up to a certain extent it will work better and faster.



Why did you choose this as your hypothesis?

Materials (Your Team’s Experiment): Plant extract, Hydrogen peroxide, water, and test tubes.



Procedure: First we have to get our enzyme by mashing up plants and creating an extract. Then we need to fill one tube with just water for the control.  Then have 3 with hydrogen peroxide that is at room temperature. Next, we need to have 3 that are heated to 50 degrees celsius and 3 that are cooled to 5 degrees celsius.



Summary: The lab was pretty easy to perform, but we got some unexpected results.  We did the controls first and got the results we expected.  Next we did our 3 heated tests and they did little to nothing at all.  We initially thought the heat would speed up and make the reaction bigger, but it seems like 50 degrees celsius was too hot for the enzyme to operate properly.  Then we did our 3 cold tests next and the results were even smaller and slower than any of them.

Data and Results:

(This section should include at least one graph!)


Control with no peroxide : No reaction.


Control with peroxide (room temp) : 4.5 cm.    4.23 minutes
50 Celsius : 1.    1.1 cm     6.24 minutes 2.    .3 cm    5.83 minutes         3.       .1 cm   5.34 minutes
                                                                      
5 Celsius : 1. .1 cm        8.9 minutes 2.     .2 cm    8.45 minutes       3.    .6 cm     9.2 minutes
                                                                             

Conclusions: In conclusion, we realized that maybe room temperature was the optimum operation temperature for the enzyme to operate since the plant we got the extract from does only grow in this time of year because the temperature.

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